کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6395307 1628471 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Kinetics of migration of colloidal particles in meat muscles in the absence and presence of a proteolytic enzyme to simulate non-motile bacteria penetration
ترجمه فارسی عنوان
سینتیک مهاجرت ذرات کلوئیدی در ماهیچه های گوشتی در غیاب و حضور یک آنزیم پروتئولیتیک برای شبیه سازی نفوذ باکتری غیر مضر
کلمات کلیدی
توزیع ذرات، شبیه سازی باکتری ها، توزیع فضایی، بافت گوشتی پروتئاز، میکروسکوپ،
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی


- Particles and defined enzyme activity can simulate behavior of non-motile bacteria.
- Particles migrated from the injection zone into the inner structure of meat.
- Particles migrated preferentially in intermediate spaces between muscle fibers.
- Cluster-like and more widespread migration of particles took place with protease.
- Apparent diffusion coefficients increased with addition of protease.

In this study, migration rate and spatial distribution of colloidal particles with and without proteolytic enzymes were studied after injection in pork loin muscle cubes for 5 days at 25 °C to simulate behavior of non-motile bacteria. Samples were monitored daily by light microscopy and confocal laser scanning microscopy and particle distribution and movement analyzed by image analysis (MATLAB®). The extent of proteolytic activity was assessed by determining the tyrosine content in muscle tissue. Results showed that particles diffused from the injection hot spot into the inner structure of the meat (1.35 ± 0.99 × 10− 12 to 2.39 ± 2.02 × 10− 12 m2/s). Upon addition of protease, the migration rate increased, i.e. the particle distribution became more widespread due to proteolysis of meat proteins reducing resistance to mass transfer. Results indicate that particles are able to diffuse into the densely packed fiber structure of meat muscles, which is contrary to the long held belief that such penetration may not occur in the absence of extensive proteolysis or mechanical damage of tissue.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Research International - Volume 75, September 2015, Pages 79-88
نویسندگان
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