HPLC-DAD-ESI/MSn characterization of environmentally friendly polyphenolic extract from Raphanus sativus L. var. “Cherry Belle” skin and stability of its red components

- Complete characterization of skin radish polyphenols by HPLC-DAD-ESI/MSn method
- Detected and identified new anthocyanins in red skin radish
- Stability assessment of red GRAS colorants potentially useful in food and cosmetic
- Developed and tested an environmentally friendly extraction method
- Estimation of CIEL*a*b* parameters of the extract mixed with mPEG

Red skin radishes (Raphanus savitus L. var. “Cherry Belle”) were extracted employing an environmentally friendly method and the obtained polyphenolic extract was characterized and studied for its applicability as a natural antioxidant colorant in nutraceutical/food and cosmetic fields. Fourteen anthocyanins, fourteen phenolic acid derivatives, and twelve flavonoids were detected. Color stability of the extract submitted to different stress conditions (thermal treatment, light and oxygen exposure) was studied both in liquid and in solid state by monitoring the absorbance at the wavelength of maximum absorption of anthocyanins when present in solutions and by estimating CIEL*a*b* parameters when mixed with mPEG. The investigation indicated a good stability at low temperatures and up to 20 °C, that was higher in the absence of oxygen and at the solid state, whereas the stability decreased at higher temperature. Therefore the extract could be used to color or improve the color of packaged food and cosmetic products to be stored at room temperature.