کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6397308 1330675 2013 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
What is inside the jar? Forensically informative nucleotide sequencing (FINS) of a short mitochondrial COI gene fragment reveals a high percentage of mislabeling in jellyfish food products
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
What is inside the jar? Forensically informative nucleotide sequencing (FINS) of a short mitochondrial COI gene fragment reveals a high percentage of mislabeling in jellyfish food products
چکیده انگلیسی
The jellyfish belonging to the Rhizostomeae order are a typical Asian seafood and are nowadays also marketed in the Western countries. The jellyfish species used for food cannot be identified due to the loss of the morphological characteristics during processing. In this work, a pre-extraction treatment consisting of desalting under running water for 48 h, followed by incubation in 400 mM EDTA solution for 1 h, was developed for products containing high concentration of salts. Moreover, considering the DNA degradation observed in both classical (CPs) and ready to eat (RE) commercial samples, five sets of primers for the amplification of fragments with different lengths belonging to the mitochondrial COI gene were designed and used to obtain the sequences from 54 reference specimens and 70 market samples. A phylogenetic analysis was then performed using the neighbor-joining method. It was found that a fragment of 142 bp was enough informative to allow identification at the species level. The results showed that most of the products were made of Nemopilema nomurai (94% of RE and 45.5% of CPs). The analysis permitted to uncover an alarming level of mislabeling which reaches 79% for CPs and 100% for ready-to-eat products.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Research International - Volume 54, Issue 2, December 2013, Pages 1383-1393
نویسندگان
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