کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6399036 1330689 2012 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Influence of encapsulated probiotics combined with pressurized longan juice on colon microflora and their metabolic activities on the exposure to simulated dynamic gastrointestinal tract
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Influence of encapsulated probiotics combined with pressurized longan juice on colon microflora and their metabolic activities on the exposure to simulated dynamic gastrointestinal tract
چکیده انگلیسی

The Simulator of the Human Intestinal Microbial Ecosystem (SHIME) was used to simulate oral administration of encapsulated Lactobacillus acidophilus LA5 or Lactobacillus casei 01 combined with pressurized longan juice. SHIME consisted of five vessels simulating the stomach, small intestine, ascending colon, transverse colon and descending colon. During the SHIME was running, colon lactobacilli and metabolized products of colon bacteria were determined. It was found that both encapsulated probiotics enabled an increase of viable colon lactobacilli. Both encapsulated probiotics combined with pressurized longan juice led to significant formation of lactic acid, propionate, butyrate and acetate. The effect of pressurized longan juice on increasing formation of lactic acid and total short-chain fatty acids as well as decreasing of ammonia formation was prominent. Both encapsulated probiotic lactobacilli along with pressurized longan juice enhanced a significant increase of bifidobacteria and a decrease of fecal coliforms and clostridia. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis confirmed that different microbial groups associated in the SHIME reactor.

► Encapsulated probiotics led to increasing colon lactobacilli community. ► Probiotics led to formation of lactic acid and short chain fatty acids (SCFA). ► Pressurized longan juice increased lactic acid and SCFA but decreased ammonia. ► Probiotics in longan juice increased beneficial but decreased pathogenic bacteria.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Research International - Volume 49, Issue 1, November 2012, Pages 133-142
نویسندگان
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