کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6400782 1330877 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An integrated method of isolating napin and cruciferin from defatted canola meal
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
An integrated method of isolating napin and cruciferin from defatted canola meal
چکیده انگلیسی


- Two major canola proteins, cruciferin and napin, have unique functional and biological properties.
- An integrated method of isolating cruciferin and napin was developed.
- The purity of cruciferin and napin were 91 and 82 g/100 g, respectively.
- The yield of cruciferin and napin were 38.6% and 12.5%, respectively.

Canola proteins are known to have great potential for use in food and non-food applications due to their nutritional, biological and functional properties. However, canola protein extraction remains a challenge, due mainly to the complexity of canola proteins and the presence a variety of undesirable compounds. An integrated method, including acidic washing (pH 4), alkaline extraction (pH 12.5), isoelectric precipitation (pH 4), and ultrafiltration, was proposed to isolate two main canola proteins: cruciferin and napin. The protein content and yield of cruciferin and napin were 91 g/100 g and 38.6%, 82 g/100 g and 12.5%, respectively. The contents of phytic acid and phenolics were 1.5 g/100 g and 1.8 g/100 g, and 0.9 g/100 g and 0.6 g/100 g, respectively, for cruciferin and napin. The emulsifying and foaming properties of cruciferin and napin were favorably comparable to previous results. The development of a simple and scalable method of canola protein isolation will facilitate further research on their food and non-food applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: LWT - Food Science and Technology - Volume 64, Issue 1, November 2015, Pages 308-315
نویسندگان
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