کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6400782 | 1330877 | 2015 | 8 صفحه PDF | دانلود رایگان |

- Two major canola proteins, cruciferin and napin, have unique functional and biological properties.
- An integrated method of isolating cruciferin and napin was developed.
- The purity of cruciferin and napin were 91Â and 82Â g/100Â g, respectively.
- The yield of cruciferin and napin were 38.6% and 12.5%, respectively.
Canola proteins are known to have great potential for use in food and non-food applications due to their nutritional, biological and functional properties. However, canola protein extraction remains a challenge, due mainly to the complexity of canola proteins and the presence a variety of undesirable compounds. An integrated method, including acidic washing (pH 4), alkaline extraction (pH 12.5), isoelectric precipitation (pH 4), and ultrafiltration, was proposed to isolate two main canola proteins: cruciferin and napin. The protein content and yield of cruciferin and napin were 91Â g/100Â g and 38.6%, 82Â g/100Â g and 12.5%, respectively. The contents of phytic acid and phenolics were 1.5Â g/100Â g and 1.8Â g/100Â g, and 0.9Â g/100Â g and 0.6Â g/100Â g, respectively, for cruciferin and napin. The emulsifying and foaming properties of cruciferin and napin were favorably comparable to previous results. The development of a simple and scalable method of canola protein isolation will facilitate further research on their food and non-food applications.
Journal: LWT - Food Science and Technology - Volume 64, Issue 1, November 2015, Pages 308-315