Inhibitory effect of polyphenols in Houttuynia cordata on advanced glycation end-products (AGEs) by trapping methylglyoxal

- Quercitrin, rutin, and cholorgenic acid were identified as major polyphenols in 50% methanol extract of Houttuynia cordata.
- H. cordata extract as well as its polyphenols scavenged MGO.
- MGO formed mono- or di-MGO adducts of quercitrin and rutin.
- H. cordata extract as well as its polyphenols inhibited AGE formation during glycation reaction.
- Methanol extract of H. cordata inhibited the formation of AGEs though trapping MGO.

The inhibitory effect of bioactive components from Houttuynia cordata (H. cordata) on advanced glycation end-products (AGEs) by trapping methylglyoxal (MGO) was investigated. MGO and its adducts of quercitrin, chlorogenic acid, rutin was analyzed by using liquid chromatography-mass spectrometry (LC-MS/MSn). The remaining MGO were 52.3, 26.7, and 9.4% for chlorogenic acid, quercitrin, and rutin, respectively and the mono- or di-MGO conjugated adducts of quercitrin and rutin were identified at 24 h of reaction. The formation of AGEs was detected through the reaction of glucose with protein by the fluorescence method. During the glycation reaction, quercitrin, rutin, and methanol extract of H. cordata decreased the production level of AGEs by 91-94.6 %. H. cordata containing chlorogenic acid, quercitrin, and rutin may have potential role in minimizing AGEs formation.