کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6401910 1330884 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Thiols in brewed coffee: Assessment by fast derivatization and liquid chromatography-high resolution mass spectrometry
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Thiols in brewed coffee: Assessment by fast derivatization and liquid chromatography-high resolution mass spectrometry
چکیده انگلیسی


- A simple, reliable method for the assessment of volatile thiols in coffee brew was proposed.
- A single-step derivatization/extraction prior LC-HRMS was optimized for coffee brew.
- Method was validated and applied to coffees with different origin.
- Seven target and nine non-target thiols were identified and quantified in coffee brew samples.

In the present paper, we present a simple, reliable, selective and sensitive method for the identification and quantification of volatile thiols at trace levels in coffee brews. A simultaneous derivatization/extraction procedure followed by liquid chromatography-electrospray high-resolution mass spectrometry is proposed and adapted to coffee brew matrix, and the performance of the method is evaluated. The linearity, sensitivity, recovery and both the intra-day and inter-day accuracy were all satisfactory. According to established identification criteria, seven target and nine non-target thiols were identified and quantified in coffee brew samples. Several of them are reported here for the first time in coffee brews, and our results are in agreement with previously reported results for coffee powder analyzed using similar analytical approach.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: LWT - Food Science and Technology - Volume 64, Issue 2, December 2015, Pages 1085-1090
نویسندگان
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