کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6402159 | 1330885 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Site-heterologous haptens and competitive monoclonal antibody-based immunoassays for pyrimethanil residue analysis in foodstuffs
ترجمه فارسی عنوان
هتپتین ها و روش های ایمنویسی مبتنی بر آنتی بادی مونوکلونال رقابتی برای تجزیه و تحلیل باقی مانده های پیریمتانیل در مواد غذایی
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
چکیده انگلیسی
Pyrimethanil is a widely used post-harvest fungicide whose residues are commonly found in fruits and vegetables. We herein report the synthesis of two new functionalized derivatives of pyrimethanil carrying an equivalent spacer arm at different positions of the molecule. The influence of the linker tethering site on the affinity of monoclonal antibodies was shown. Moreover, the development of competitive immunoassays in different formats and with high sensitivity to the target analyte - IC50 values below 0.3 μg/L - is described. Optimized assays were characterized by the determination of the limit of quantification, trueness, and precision using water-diluted QuEChERS extracts of fruits and vegetables fortified with pyrimethanil. Finally, in-field treated and blind spiked strawberry, tomato, and cucumber samples were analyzed by the developed immunoassays and a reference chromatographic method. Using Deming regression analysis, we show that results from both methodologies were statistically comparable.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: LWT - Food Science and Technology - Volume 63, Issue 1, September 2015, Pages 604-611
Journal: LWT - Food Science and Technology - Volume 63, Issue 1, September 2015, Pages 604-611
نویسندگان
Francesc A. Esteve-Turrillas, Josep V. Mercader, Consuelo Agulló, Antonio Abad-Somovilla, Antonio Abad-Fuentes,