کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6402465 1330888 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
NMR measurement of bacteria death kinetics during heat stress
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
NMR measurement of bacteria death kinetics during heat stress
چکیده انگلیسی


- 1H NMR method found to rapidly quantify the heat stress death kinetics.
- Ratio of methylene and methyl peaks directly proportional to death percentage.
- Method rapid, in situ, minimal preparation and no chemical agents required.

Modelling the thermal processing of food matrices incorporating microorganisms requires heat stress death kinetics. It is demonstrated that proton nuclear magnetic resonance (1H NMR) is a rapid means of quantifying the heat stress death kinetics of a human probiotic bacterium (Bifidobacterium animalis subsp. lactis DR10™ or strain HN019). It was found that the ratio of the methylene (CH2) resonance at 1.2 ppm to the methyl (CH3) resonance at 0.9 ppm signal intensity as observed by 1H NMR was directly proportional to the percentage of death of a population of cells during heat stress. The derived death kinetic rate constants for a series of heating temperatures showed excellent correlation to the Arrhenius equation. The method has several advantages over existing methods for measuring bacterial death kinetics, as it can be carried out in situ with minimal preparation and without the addition of chemical agents.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: LWT - Food Science and Technology - Volume 60, Issue 2, Part 1, March 2015, Pages 876-880
نویسندگان
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