کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
640290 | 1456965 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Optimization and miniaturization of aqueous two phase systems for the purification of recombinant human immunodeficiency virus-like particles from a CHO cell supernatant
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تصفیه و جداسازی
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چکیده انگلیسی
In this work, ATPE conditions for the purification of a Human Immunodeficiency Virus (HIV) VLP were screened and optimized in mL scale batch conditions. Polyethylene glycol (PEG)-salt (potassium phosphate, ammonium sulfate and trisodium citrate) and polymer-polymer (PEG-dextran) systems were investigated, among which the PEG-ammonium sulfate system demonstrated the higher partition coefficient (K = 4.4). This parameter was then compared with the obtained in a continuous microfluidic setting, performed by flowing both immiscible phases through a 100 width Ã 20 μm wide microchannel. The batch optimization results showed good agreement with the continuous miniaturized extraction, both in terms of K (K = 3.9 in microfluidic scale) and protein purity. These novel findings show that PEG-ammonium sulfate ATPE is a promising system for primary HIV-VLP recovery and demonstrate the potential of a miniaturized ATPE for massive parallelization (scale-out) at the preparative scale or integrated in analytical miniaturized systems.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Separation and Purification Technology - Volume 154, 5 November 2015, Pages 27-35
Journal: Separation and Purification Technology - Volume 154, 5 November 2015, Pages 27-35
نویسندگان
M.J. Jacinto, R.R.G. Soares, A.M. Azevedo, V. Chu, A. Tover, J.P. Conde, M.R. Aires-Barros,