Effects of skipjack roe protein hydrolysate on properties and oxidative stability of fish emulsion sausage

- Skipjack roe protein hydrolysate (SRPH) acted as emulsifier in emulsion sausage.
- Addition of SRPH improved textural properties of fish emulsion sausage.
- SRPH could retard lipid oxidation in emulsion sausage during the refrigerated stroage.

Effects of skipjack roe protein hydrolysate (SRPH) at various levels (0-3 g/100 g) on properties and oxidative stability of emulsion sausage from broadhead catfish (Clarias macrocephalus) fortified with skipjack tuna roe lipids were investigated. The addition of SRPH increased hardness, cohesiveness and resilience of sausage (p < 0.05). Finer fat globules were visualised in the sample added with SRPH at higher amounts. Nevertheless, the incorporation of SRPH at all levels had no impact on likeness of sausages. SRPH was shown to retard lipid oxidation of sausage during extended storage of 12 days as evidenced by the lower peroxide value (PV) and thiobarbituric acid-reactive substances (TBARS), in comparison with the control. After 12 days, the sausage with 3 g/100 g SRPH had the retained docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), accounting more than 80%. Addition of SRPH had no effect on the organoleptic properties but could prevent the development of rancidity. Nevertheless, it showed no pronounced impact on microbial growth. SRPH could therefore be used as a natural antioxidative emulsifier in cooked fish emulsion sausage.