Micelle-mediated extraction of chlorogenic acid from Morus laevigata W. leaves

Chlorogenic acid (CGA) is a phenolic antioxidant compound with medicinal activities especially as promising precursor for the development of medicines that can resist AIDS virus HIV. This study explores the micelle-mediated extraction of CGA from Morus laevigata W. leaves which is superior to conventional methods involving organic or organo-aqueous solvents. In this new method, sodium dodecylsulfate (SDS) surfactant solution was used as efficient extraction medium in combination with microwave irradiation to shorten the extraction time. Crude extract of CGA with SDS solution as solvent was purified by ethyl acetate at high ionic strength followed by washing with hexane: chloroform mixture. Quantitation of CGA was carried out on liquid chromatography with diode array detection. The optimum extraction of 2152 mg/100 g CGA from freeze dried M. laevigata W. leaves was achieved using 0.1 M of SDS at pH 1.0 with extraction time of 10 s and microwave power of 50 W. Best results were observed when 0.2 g of plant material was extracted with 20 mL of solvent. CGA obtained from M. laevigata W. leaves was compared with pure standard of CGA, and found to be 95.9% pure.

Figure optionsDownload as PowerPoint slideResearch highlights▶ Extraction of chlorogenic acid is performed with surfactant solution. ▶ Anionic surfactant, SDS, at pH 1.0 was found best solvent of all the surfactants studied. ▶ Synergistic effect of microwave irradiation expedite the extractions. ▶ Chlorogenic acid can be purified from surfactant solutions by micellar precipitation.