کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
643150 | 884358 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid concentration of protein solution by a crossflow electro-ultrafiltration process
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تصفیه و جداسازی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
An electro-ultrafiltration process was proposed and developed for concentrating protein solution rapidly by applying an electric field and employing a membrane with a higher nominal molecular weight cut-off (NMWCO) than the molecular weight of protein to be concentrated. In this process, the high NMWCO membrane was used to achieve a high permeate flux while the adoption of an electric field was used to obtain a high rejection of the target protein by manipulating the electrophoretic effect. Bovine serum albumen (BSA) was used as a model protein, and 50 and 100 kDa NMWCO polysulphone (PS) membranes were used for BSA concentration. The effects of membrane NMWCO, electric field strength, feed concentration on permeate flux and protein rejection were examined. The experimental results showed that the concentration time required for a target protein concentration could be significantly shortened when an electric field was applied and a membrane with higher NMWCO was used. When a 3000 V mâ1 electric field was applied and the 100 kDa NMWCO polysulphone (PS) membrane was used, the concentration time for concentrating 0.5 g Lâ1 BSA solution to around 1.0 g Lâ1 decreased by 80% as compared with the process with a 50 kDa membrane without electric field, suggesting that this electro-ultrafiltration concentration process is a viable alternative to conventional ultrafiltration process and a promising method for concentrating dilute protein solutions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Separation and Purification Technology - Volume 73, Issue 2, 18 June 2010, Pages 310-318
Journal: Separation and Purification Technology - Volume 73, Issue 2, 18 June 2010, Pages 310-318
نویسندگان
Weijie Song, Yi Su, Xiangrong Chen, Luhui Ding, Yinhua Wan,