کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6449740 1415936 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Full length articleOne step fabrication of hydrogel microcapsules with hollow core for assembly and cultivation of hepatocyte spheroids
ترجمه فارسی عنوان
مقاله طول کامل یک گام ساخت میکروکپسولهای هیدروژل با هسته توخالی برای مونتاژ و کشت اسپری های هپاتوسیت
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی

3D hepatic microtissues can serve as valuable liver analogues for cell-based therapies and for hepatotoxicity screening during preclinical drug development. However, hepatocytes rapidly dedifferentiate in vitro, and typically require 3D culture systems or co-cultures for phenotype rescue. In this work we present a novel microencapsulation strategy, utilizing coaxial flow-focusing droplet microfluidics to fabricate microcapsules with liquid core and poly(ethylene glycol) (PEG) gel shell. When entrapped inside these capsules, primary hepatocytes rapidly formed cell-cell contacts and assembled into compact spheroids. High levels of hepatic function were maintained inside the capsules for over ten days. The microencapsulation approach described here is compatible with difficult-to-culture primary epithelial cells, allows for tuning gel mechanical properties and diffusivity, and may be used in the future for high density suspension cell cultures.Statement of SignificanceOur paper combines an interesting new way for making capsules with cultivation of difficult-to-maintain primary epithelial cells (hepatocytes). The microcapsules described here will enable high density suspension culture of hepatocytes or other cells and may be used as building blocks for engineering tissues.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Biomaterialia - Volume 50, 1 March 2017, Pages 428-436
نویسندگان
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