کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6450438 1361277 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Regular articleMonolithic biocatalytic systems with enhanced stabilities constructed through biomimetic silicification-induced enzyme immobilization on rGO/FeOOH hydrogel
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Regular articleMonolithic biocatalytic systems with enhanced stabilities constructed through biomimetic silicification-induced enzyme immobilization on rGO/FeOOH hydrogel
چکیده انگلیسی


- Stable monolithic biocatalytic systems were designed and constructed.
- Enzyme was immobilized on rGO-based hydrogel surface via biomimetic silicification.
- Structure of the biocatalytic systems could be facilely tailored.
- The biocatalytic systems showed structure-dependent catalytic activity.
- Enhanced pH/thermal/recycling/storage stabilities were achieved.

In this study, we present a green and facile method of utilizing biomimetic silicification to trigger enzyme immobilization on the surface of the rGO/FeOOH hydrogel for constructing stable monolithic biocatalytic systems. In brief, the rGO/FeOOH hydrogel is firstly prepared through metal ion-induced reduction/assembly of graphene oxide (GO) nanosheets, which is then utilized to adsorb cationic polyethyleneimine (PEI). This cationic PEI, as the mineralization-inducing agent, catalyzes the condensation of silicate to form silica (biomimetic silicification) on the rGO surface, where enzyme is simultaneously entrapped. The resultant rGO/FeOOH/silica hydrogel shows an extraordinary three-dimensional (3D) porous structure. The silica content on the rGO surface can be facilely tailored through changing the silica precursor concentration. Combined with monolithic macroscale of the rGO/FeOOH/silica hydrogel, the acquired monolithic biocatalytic systems display easy recyclability and elevated pH/thermal/recycling/storage stabilities during the catalytic production of 6-aminopenicillanic acid (6-APA) in comparison to enzyme in free form and enzyme adsorbed on rGO/FeOOH hydrogel. Notably, the activity can be retained up to 93.3% of its initial activity after 11 reaction cycles for our biocatalytic systems.

A biomimetic silicification method was explored to accomplish enzyme immobilization on the rGO/FeOOH hydrogel surface for constructing stable monolithic biocatalytic systems.260

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 117, Part B, 15 January 2017, Pages 52-61
نویسندگان
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