کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6450438 | 1361277 | 2017 | 10 صفحه PDF | دانلود رایگان |
- Stable monolithic biocatalytic systems were designed and constructed.
- Enzyme was immobilized on rGO-based hydrogel surface via biomimetic silicification.
- Structure of the biocatalytic systems could be facilely tailored.
- The biocatalytic systems showed structure-dependent catalytic activity.
- Enhanced pH/thermal/recycling/storage stabilities were achieved.
In this study, we present a green and facile method of utilizing biomimetic silicification to trigger enzyme immobilization on the surface of the rGO/FeOOH hydrogel for constructing stable monolithic biocatalytic systems. In brief, the rGO/FeOOH hydrogel is firstly prepared through metal ion-induced reduction/assembly of graphene oxide (GO) nanosheets, which is then utilized to adsorb cationic polyethyleneimine (PEI). This cationic PEI, as the mineralization-inducing agent, catalyzes the condensation of silicate to form silica (biomimetic silicification) on the rGO surface, where enzyme is simultaneously entrapped. The resultant rGO/FeOOH/silica hydrogel shows an extraordinary three-dimensional (3D) porous structure. The silica content on the rGO surface can be facilely tailored through changing the silica precursor concentration. Combined with monolithic macroscale of the rGO/FeOOH/silica hydrogel, the acquired monolithic biocatalytic systems display easy recyclability and elevated pH/thermal/recycling/storage stabilities during the catalytic production of 6-aminopenicillanic acid (6-APA) in comparison to enzyme in free form and enzyme adsorbed on rGO/FeOOH hydrogel. Notably, the activity can be retained up to 93.3% of its initial activity after 11 reaction cycles for our biocatalytic systems.
A biomimetic silicification method was explored to accomplish enzyme immobilization on the rGO/FeOOH hydrogel surface for constructing stable monolithic biocatalytic systems.260
Journal: Biochemical Engineering Journal - Volume 117, Part B, 15 January 2017, Pages 52-61