کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6452657 1361476 2017 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Original Research ArticleProbing the metabolic phenotype of breast cancer cells by multiple tracer stable isotope resolved metabolomics
ترجمه فارسی عنوان
بررسی فنوتیپ متابولیک سلول های سرطانی پستان با متابولیکی از طریق چند تست ثابت ایزوتوپ
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


- Nutrient utilization in different breast cancer cell lines is determined by stable isotope resolved metabolomics.
- There are clear metabolic differences between estrogen -dependent and triple negative cell lines, especially in lipid synthesis.
- Differences in metabolic phenotypes are recapitulated in orthotopic mouse xenografts.

Breast cancers vary by their origin and specific set of genetic lesions, which gives rise to distinct phenotypes and differential response to targeted and untargeted chemotherapies. To explore the functional differences of different breast cell types, we performed Stable Isotope Resolved Metabolomics (SIRM) studies of one primary breast (HMEC) and three breast cancer cells (MCF-7, MDAMB-231, and ZR75-1) having distinct genotypes and growth characteristics, using 13C6-glucose, 13C-1+2-glucose, 13C5,15N2-Gln, 13C3-glycerol, and 13C8-octanoate as tracers. These tracers were designed to probe the central energy producing and anabolic pathways (glycolysis, pentose phosphate pathway, Krebs Cycle, glutaminolysis, nucleotide synthesis and lipid turnover). We found that glycolysis was not associated with the rate of breast cancer cell proliferation, glutaminolysis did not support lipid synthesis in primary breast or breast cancer cells, but was a major contributor to pyrimidine ring synthesis in all cell types; anaplerotic pyruvate carboxylation was activated in breast cancer versus primary cells. We also found that glucose metabolism in individual breast cancer cell lines differed between in vitro cultures and tumor xenografts, but not the metabolic distinctions between cell lines, which may reflect the influence of tumor architecture/microenvironment.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 43, Part B, September 2017, Pages 125-136
نویسندگان
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