کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6452886 44948 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Full length ArticleTobacco as platform for a commercial production of cyanophycin
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Full length ArticleTobacco as platform for a commercial production of cyanophycin
چکیده انگلیسی


- Production of cyanophycin in the commercial N. tabacum cultivars BG and VG.
- Cyanophycin yielded up to 9.4% per dry weight (DW) in hyrbids of transgenic pH .
- Cyanophycin yielded up to 8.8% DW in T0 transformants.
- Yields were homogenous in T0 and T1 plants of BG.
- The polymer is stable in planta as well as during silage.

Cyanophycin (CP) is a proteinogenic polymer that can be substituted for petroleum in the production of plastic compounds and can also serve as a source of valuable dietary supplements. However, because there is no economically feasible system for large-scale industrial production, its application is limited. In order to develop a low-input system, CP-synthesis was established in the two commercial Nicotiana tabacum (N. tabacum) cultivars 'Badischer Geudertheimer' (BG) and 'Virginia Golta' (VG), by introducing the cyanophycin-synthetase gene from Thermosynecchococcus elongatus BP-1 (CphATe) either via crossbreeding with transgenic N. tabacum cv. Petit Havana SR1 (PH) T2 individual 51-3-2 or by agrobacterium-mediated transformation. Both in F1 hybrids (max. 9.4% CP/DW) and T0 transformants (max. 8.8% CP/DW), a substantial increase in CP content was achieved in leaf tissue, compared to a maximum of 1.7% CP/DW in PH T0 transformants of Hühns et al. (2008). In BG CP, yields were homogenous and there was no substantial difference in the variation of the CP content between primary transformants (T0), clones of T0 individuals, T1 siblings and F1 siblings of hybrids. Therefore, BG meets the requirements for establishing a master seed bank for continuous and reliable CP-production. In addition, it was shown that the polymer is not only stable in planta but also during silage, which simplifies storage of the harvest prior to isolation of CP.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: New Biotechnology - Volume 33, Issue 6, 25 December 2016, Pages 842-851
نویسندگان
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