Isolation of PCR ready-human DNA using copper nanoparticles from skeletal remains

- A novel approach of PCR ready-human DNA extraction method from skeletal remains.
- Rapid, cost effective, sensitive and non-hazardous method.
- Required a very small quantities of blood and skeletal remains.
- Does not require hazardous organic solvents or expensive enzyme treatment.
- First time, one-step green rout synthesized copper nanoparticles (CuNPs) is applied.

Present study represents a novel approach of PCR ready-human DNA extraction method from skeletal remains using copper nanoparticles (CuNPs) for personnel identification. To achieve rapid, cost effective, sensitive and non-hazardous method for DNA extraction we utilized CuNPs synthesized using microwave. The applicability of this approach was first tested in blood samples and afterwards, this system was extended to skeletal remains' samples also. This method yields good quality DNA that are ready for PCR reactions from small quantities of blood and skeletal remains. Consequently, even small quantities of nanoparticles could be potentially utilized for a highly efficient isolation of DNA from skeletal remains as well as from ancient archaeological samples. The present method has the advantages that it is quick with high yield, inexpensive, robust, environment friendly and does not require use of hazardous organic solvents.

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