Carboxymethyl cellulose assisted preparation of water-processable halloysite nanotubular composites with carboxyl-functionalized multi-carbon nanotubes for simultaneous voltammetric detection of uric acid, guanine and adenine in biological samples

• Water-processable HNTs hybrid nanotubes with fMWCNTs were prepared by CMC assist.
• CMC-HNTs-fMWCNTs/GCE was characterized with good properties for sensing application.
• UA, G and A in real samples were electrochemically detected by CMC-HNTs-fMWCNTs/GCE.

Uric acid (UA), a waste product of purine derivatives catabolism, is one of the main culprits of gout. In this paper, a simple and sensitive method for the simultaneous voltammetric detection of UA and the most common purine derivatives like guanine (G) and adenine (A) in human serum and lettuce samples was successfully realized using glassy carbon electrode (GCE) modified with the water-processable nanotubular composites consisting of both halloysite nanotubes (HNTs) and carboxyl-functionalized multi-walled carbon nanotubes (fMWCNTs) that were well dispersed by carboxymethyl cellulose (CMC). A water-processable HNTs hybrid nanotubes with fMWCNTs were successfully prepared using CMC assist, and the CMC-HNTs-fMWCNTs composites were characterized by scanning electron microscopy, fourier-transform infrared spectroscopy, transmission electron microscope, electrochemical impedance spectroscopy, cyclic voltammetry and chronocoulometry. CMC-HNTs-fMWCNTs/GCE displayed enhanced electrode stability in water, effective surface area, adhesive property and adsorption capacity, and electrocatalytic activity for the oxidation of UA, G and A, which could simultaneously detected three purine derivatives in linear ranges from 1 to 130 μM with a limit of detection (LOD) of 0.5 μM for UA, 0.05 to 9 μM with LOD of 0.018 μM for G, and 0.3 to 25 μM with LOD of 0.19 μM for A, respectively. The as-fabricated sensor displayed high sensitivity, low detection limit, good sensing stability, remarkable feasibility, and satisfactory practicality.

The important sources of UA, G and A and their simultaneous determination using CMC-HNTs-fMWCNTs composites modified electrode.Figure optionsDownload high-quality image (107 K)Download as PowerPoint slide