Sensitive electrochemical sensor for simultaneous determination of uric acid and xanthine in human biological fluids based on the nano-boron doped ceria modified glassy carbon paste electrode

- Sensitive electrochemical sensor for simultaneous determination of uric acid (UA) and xanthine (XA)
- The nano-B-CeO2/GCPE sensor showed high electrocatalytic activity and sensitivity toward the oxidation of UA and XA.
- Sensing of UA and XA in the presence of ascorbic acid and dopamine
- Analytical application of the nano-B-CeO2/GCPE was successfully applied for detection of UA and XA in biological fluids.
- The modified electrode exhibited considerable sensitivity, stability and reproducibility.

A sensitive and selective electrochemical method was developed for simultaneous determination of uric acid and xanthine using the nano boron doped ceria modified glassy carbon paste electrode (nano-B-CeO2/GCPE). The proposed sensor exhibited an excellent catalytic activity, high sensitivity and good selectivity toward the oxidation of uric acid and xanthine. The potential mutual interference of uric acid (UA), xanthine (XA), dopamine (DA) and ascorbic acid (AA) were examined indicting that their oxidation take place independently at the nano-B-CeO2/GCPE sensor. For simultaneous detection by synchronous change of the concentration of UA and XA, the linear ranges were 0.42-11.87 μM and 0.07-2.02 μM with detection limits of 5.39 × 10− 9 M and 2.36 × 10− 9 M, respectively. The proposed method was successfully applied to simultaneous determination of UA and XA in human urine and serum samples with satisfactory results.

Simultaneous electrochemical determination of structurally similar purine derivatives, UA and XA using nano-B-CeO2/GCPE in biological fluids was investigated. The potential mutual interference of uric acid (UA), xanthine (XA), dopamine (DA) and ascorbic acid (AA) were examined indicting that their oxidation take place independently at the nano-B-CeO2/GCPE sensor.314