Stabilization and detection of hydrophylloquinone as di-O-methyl derivative

â¿¢Alternative procedure to identify the hydrophylloquinone.â¿¢A method to stabilize hydrophylloquinone by di-O-methylation was developed.â¿¢Chromatographic methods to detect di-O-methyl hydrophylloquinone were developed.â¿¢Phylloquinone and di-O-methyl hydrophylloquinone were characterized by EI, HRESI+, 1D and 2D NMR.

Phylloquinone is a redox active naphthoquinone involved in electron transport in plants. The function of this reduced form remains unclear due to its instability, which has precluded detection. Herein, a simple method that permits the stabilization of the reduced form of phylloquinone by di-O-methylation and HPLC detection is described.