کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6481689 1540227 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of chitosan hydrochloride-alginate as enteric micro-probiotic-carrier with dual protective barriers
ترجمه فارسی عنوان
ارزیابی هیدروکلراید آلژینات کیتوزان به عنوان پروتئین میکرو پروبیوتیک غضروفی با موانع محافظ دوگانه
کلمات کلیدی
مجتمع های پلی الکترولیتی، میکرو پروبیوتیک حامل، میکروکنپسوله کردن،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی

In this study, the cells-free and cells-loaded chitosan hydrochloride-alginate (CHC-Alg) microcapsules were firstly fabricated with polyelectrolyte complexes via an orifice-polymerization method. Scanning electron microscope images showed that the CHC-Alg microcapsules had a typical shell-core structure and the model probiotic cells (Bacillus licheniformis) were embedded in the core in cells-loaded microcapsules. The microcapsules prepared had good thermal stability and moisture property (3.89%). Cells survival and release studies showed that the number of probiotic cells released from the cells-loaded microcapsules (approx. 6.36 log CFU ml−1) was 6.19 log CFU ml−1 when they were performed in the simulated gastric fluid (SGF, pH 2.0) for 1 h and subsequently in the simulated intestinal fluid (SIF, 0.3%) for 4 h. The CHC-Alg microcapsules with favorable swelling performances were helpful to permeate the harsh acid to protect the cells in the SGF (pH 2.0). The CHC-Alg microcapsules effectively protected the model probiotic cells, which was attributed to the “dual protective barriers” of the shell-core structure, that is, the primary barrier of the Alg hydrogel layer formed with a compact polymer matrix and the secondary barrier of the PEC film formed on the surface. The microcapsules prepared could be used as an enteric micro-probiotic-carrier for designing potential probiotic delivery systems.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 93, Part A, December 2016, Pages 665-671
نویسندگان
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