کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6482185 | 1415979 | 2018 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Coordination of GMP ligand with Cu to enhance the multiple enzymes stability and substrate specificity by co-immobilization process
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Enhancing activity of multiple enzymes by co-localization are key topics in bionanotechnology. Herein, we introduce a facile co-immobilization of glucose oxidase (GOx) and horseradish peroxidase (HRP) in the matrix of Cu (copper) and GMP (guanosine 5â²-monophosphate). The multienzyme system by co-immobilizing GOx&HRP observed with high catalytic efficiency and stability compared with free GOx&HRP enzymes. GOx&HRP@Cu/GMP stability was observed 40% increased at pH 3 and thermal stability more than 70% determined at 90 áµC. The storage stability was sustained 8 times higher than free enzymes after thirteen days and reusability after reacting eight times with fresh substrate retains 90% relative activity. Further, GOx&HRP@Cu/GMP demonstrated an excellent selectivity in the development of glucose biosensor and exhibited good resistance against the protease enzymes and organic solvents. The obtained results emphasize that co-immobilization of GOx&HRP in the matrix of Cu/GMP could be an efficient protocol for the biocatalyst encapsulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 136, 15 August 2018, Pages 102-108
Journal: Biochemical Engineering Journal - Volume 136, 15 August 2018, Pages 102-108
نویسندگان
Amjad Hussain Memon, Runsheng Ding, Qipeng Yuan, Hao Liang, Yun Wei,