کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6483858 1415977 2018 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Perspective: Solidifying the impact of cell-free synthetic biology through lyophilization
ترجمه فارسی عنوان
چشم انداز: تثبیت اثر زیست شناسی مصنوعی سلولی از طریق لیوفیلیزاسیون
کلمات کلیدی
زیست شناسی مصنوعی بدون سلول، مقاله مبتنی بر، سنسورها، تشخیص تولید درمانی قابل حمل، واکنش های آزاد بدون انجماد،
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Cell-free synthetic biology is an exciting and new branch in the field of synthetic biology. Based on in vitro transcription and translation systems, this application-focused domain builds on decades of cell-free biochemistry and protein expression to operate synthetic gene networks outside of cellular environments. This has brought new and perhaps even unexpected advantages. Chief among these is the ability to operate genetically encoded tools in a sterile and abiotic format. Recent work has extended this advantage by freeze-drying these cell-free systems into dried pellets or embedded paper-based reactions. Taken together, these new ideas have solved the longstanding challenge of how to deploy poised synthetic gene networks in a biosafe mode outside of the laboratory. There is significant excitement in the potential of this newfound venue and the community has begun to extend proof-of-concept demonstrations in important and creative ways. Here I explore these new efforts and provide my thoughts on the challenges and opportunities ahead for freeze-dried, cell-free synthetic biology.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 138, 15 October 2018, Pages 91-97
نویسندگان
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