کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6484071 | 158 | 2014 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Internal and external mass transfer limitations on the activity of immobilised acid urease derivatives differing in enzyme loading
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Internal and external mass transfer limitations on the activity of immobilised acid urease derivatives differing in enzyme loading Internal and external mass transfer limitations on the activity of immobilised acid urease derivatives differing in enzyme loading](/preview/png/6484071.png)
چکیده انگلیسی
The specific activity of several Eupergit® C 250 L immobilised acid urease derivatives with enzyme loadings (YP/B) of 48-171Â mg of bovine serum albumin equivalent (BSAE) per gram of dry support (ds) in a model wine solution was satisfactorily reconstructed using the simultaneous film and intraparticle diffusion and Michaelis-Menten kinetic model by assuming that the immobilisation procedure did not alter the intrinsic kinetic parameters of free acid urease. The behaviour of crunched biocatalysts resulted to be practically coincident with that of the free enzyme, while that of the whole beads was affected by diffusional effects. The model prediction capability was also established in long-time reaction tests using two other biocatalysts at different dry bead concentrations (cBd). The effect of YP/B was finally simulated in the pseudo-1st order regime in the presence of a stagnant or highly-stirred medium. At the limiting Sherwood number of 2.0 and YP/BÂ >Â 48Â mg BSAE gâ1 ds, the calculated specific activity was greatly affected by external mass transfer. To help designing stirred bioreactors, the substrate reduction to be achieved in a given time interval was empirically related to cBd and YP/B.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 82, 15 January 2014, Pages 22-33
Journal: Biochemical Engineering Journal - Volume 82, 15 January 2014, Pages 22-33
نویسندگان
Nadia Bortone, Marcello Fidaleo, Mauro Moresi,