Synthesis of prebiotic galactooligosaccharides from lactose using bifidobacterial β-galactosidase (BbgIV) immobilised on DEAE-Cellulose, Q-Sepharose and amino-ethyl agarose

- The bifidobacterial β-galactosidase BbgIV was immobilised on various supports.
- The immobilisation yield exceeded 90% using Q-Sepharose and DEAE-Cellulose.
- Immobilisation significantly increased the operational stability of BbgIV up to 55 °C.
- Synthesis of galactooligosaccharides was further improved using immobilised BbgIV.
- A robust process including 6 successive synthesis batches was developed.