کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6484102 | 169 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Kinetic modeling and implementation of superior process strategies for β-galactosidase production during submerged fermentation in a stirred tank bioreactor
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کلمات کلیدی
μmaxXmaxPmaxKLA - ارتش آزادیبخشβ-galactosidase - بتا گالاکتوزیدازFed-batch fermentation - تخمیر بذر فدرالSubmerged fermentation - تخمیر زیرزمینیTime (h) - زمان (ساعت)Volumetric mass transfer coefficient - ضریب انتقال جرمی حجمیLactobacillus acidophilus - لاکتوباسیلوس اسیدوفیلوسStirred tank bioreactor - مخلوط بیوراکتور مخزن
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This paper reports development and implementation of superior fermentation strategies for β-galactosidase production by Lactobacillus acidophilus in a stirred-tank bioreactor. Process parameters (aeration and agitation) were optimized for the process by application of Central Composite Design. Aeration rate of 0.5 vvm and agitation speed of 250 rpm were most suitable for β-galactosidase production (2001.2 U/L). Further improvement of the operation in pH controlled environment resulted in 2135 U/L of β-galactosidase with productivity of 142.39 U/L h. Kinetic modeling for biomass and enzyme production and substrate utilization were carried out at the aforementioned pH controlled conditions. The logistic regression model (X0 = 0.01 g/L; Xmax = 2.948 g/L; μmax = 0.59/h; R2 = 0.97) was used for mathematical interpretation of biomass production. Mercier's model proved to be better than Luedeking-Piret model in describing β-galactosidase production (P0 = 0.7942 U/L; Pmax = 2169.3 U/L; Pr = 0.696/h; R2 = 0.99) whereas the latter was more efficient in mathematical illustration of lactose utilization (m = 0.187 g/g h; Yx/s = 0.301 g/L; R2 = 0.98) among the two used in this study. Strategies like fed-batch fermentation (3694.6 U/L) and semi-continuous fermentation (5551.9 U/L) further enhanced β-galactosidase production by 1.8 and 2.8 fold respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 77, 15 August 2013, Pages 49-57
Journal: Biochemical Engineering Journal - Volume 77, 15 August 2013, Pages 49-57
نویسندگان
Huzaifa S. Choonia, S.S. Lele,