کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6484107 | 169 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
NAD+ regeneration in a microreactor using permeabilized baker's yeast cells
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
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چکیده انگلیسی
In this study regeneration of coenzyme NAD+ was carried out in a microreactor by acetaldehyde reduction to ethanol using enzyme alcohol dehydrogenase (ADH). Suspended and immobilized whole permeabilized baker's yeast cells were used as the source of the ADH enzyme. A 65.3% conversion of NADH was achieved with suspended permeabilized baker's yeast cells for a residence time of Ï = 36 s and equimolar concentration of substrates (ci,NADH = 5.5 mmol/dm3, ci,acetaldehyde = 5.5 mmol/dm3). When working with immobilized cells, conversion achieved for the same residence time was 10 fold lower. When permeabilized baker's yeast cells were used for coenzyme regeneration process was stabile for 6 days of continuous operation which makes this system a good alternative for coenzyme regeneration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 77, 15 August 2013, Pages 88-96
Journal: Biochemical Engineering Journal - Volume 77, 15 August 2013, Pages 88-96
نویسندگان
Anita Å aliÄ, Petra Faletar, Bruno ZeliÄ,