Regioselectivity-driven evolution of CYP102D1 for improved synthesis of 3′-ortho-dihydroxyisoflavone

- Regioselective hydroxylation of daidzein.
- 3′-Specific hydroxylation drive evolution of CYP102D1.
- Protein engineering through O-dealkylation based HTS assay.
- Site-specific saturation mutagenesis.
- Structural understanding of protein mutagenesis.