کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6494343 | 44806 | 2015 | 43 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An ensemble-guided approach identifies ClpP as a major regulator of transcript levels in nitric oxide-stressed Escherichia coli
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The importance of NOâ to immunity is highlighted by the diversity of pathogens that require NOâ-defensive systems to establish infections. Proteases have been identified to aid pathogens in surviving macrophage attack, inspiring us to investigate their role during NOâ stress in Escherichia coli. We discovered that the elimination of ClpP largely impaired NOâ detoxification by E. coli. Using a quantitative model of NOâ stress, we employed an ensemble-guided approach to identify the underlying mechanism. Iterations of in silico analyses and corresponding experiments identified the defect to result from deficient transcript levels of hmp, which encodes NOâ dioxygenase. Interestingly, the defect was not confined to hmp, as ÎclpP imparted widespread perturbations to the expression of NOâ-responsive genes. This work identified a target for anti-infective therapies based on disabling NOâ defenses, and demonstrated the utility of model-based approaches for exploring the complex, systems-level stress exerted by NOâ.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 31, September 2015, Pages 22-34
Journal: Metabolic Engineering - Volume 31, September 2015, Pages 22-34
نویسندگان
Jonathan L. Robinson, Mark P. Brynildsen,