کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6494402 | 44804 | 2015 | 45 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Efficient and specific transporters may enhance pentose uptake and metabolism by Saccharomyces cerevisiae. Eight heterologous sugar transporters were characterized in S. cerevisiae. The transporter Mgt05196p from Meyerozyma guilliermondii showed the highest xylose transport activity among them. Several key amino acid residues of Mgt05196p were suggested by structural and sequence analysis and characterized by site-directed mutagenesis. A conserved aromatic residue-rich motif (YFFYY, position 332-336) in the seventh trans-membrane span plays an important role in d-xylose transport activity. The phenyl ring of the residue at position 336 may take the function to prevent d-xylose from escaping during uptake. F432A and N360S mutations enhanced the d-xylose transport activities of Mgt05196p. Furthermore, mutant N360F specifically transported d-xylose without any glucose-inhibition, high lighting its potential application in constructing glucose-xylose co-fermentation strains for biomass refining.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 30, July 2015, Pages 79-88
Journal: Metabolic Engineering - Volume 30, July 2015, Pages 79-88
نویسندگان
Chengqiang Wang, Xiaoming Bao, Yanwei Li, Chunlei Jiao, Jin Hou, Qingzhu Zhang, Weixin Zhang, Weifeng Liu, Yu Shen,