کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6494476 44809 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Regulatory effects on central carbon metabolism from poly-3-hydroxybutryate synthesis
ترجمه فارسی عنوان
اثرات تنظیم کننده بر متابولیسم مرکزی کربن از سنتز پلی-3-هیدروکسی بوراتیت
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
To understand the regulatory mechanisms that resulted in these macroscopic changes, we used a flux balance analysis model to analyze intracellular redox conditions. Our model shows that under N-limited conditions, synthesis of PHB creates excess reducing equivalents. Cells, under these conditions, secrete more reduced metabolites in order to recycle reducing equivalents. By switching to a more oxidized substrate (gluconate) that decreased excess reducing equivalents, PHB flux yield increased 1.6 fold compared to glucose-fed fermentations. High flux of PHB (~1.2 mmol/g DCW h) was maintained under these steady-state, oxidized conditions. These results imply redox imbalance is a driving force in industrial production of PHB, and substrates that are more oxidized than glucose can increase productivity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 28, March 2015, Pages 180-189
نویسندگان
, ,