کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6494729 | 44822 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Design, construction and characterisation of a synthetic promoter library for fine-tuned gene expression in actinomycetes
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Design, construction and characterisation of a synthetic promoter library for fine-tuned gene expression in actinomycetes Design, construction and characterisation of a synthetic promoter library for fine-tuned gene expression in actinomycetes](/preview/png/6494729.png)
چکیده انگلیسی
We developed a synthetic promoter library for actinomycetes based on the â10 and â35 consensus sequences of the constitutive and widely used ermEp1 promoter. The sequences located upstream, in between and downstream of these consensus sequences were randomised using degenerate primers and cloned into an integrative plasmid upstream of the gusA reporter gene. Using this system, we created promoters with strengths ranging from 2% to 319% compared with ermEp1. The strongest synthetic promoter was used in a proof-of-principle approach to achieve the overexpression of a natural type III polyketide synthase. We observed high correlation between the number of gusA reporter gene RNA-Seq reads and the GusA reporter protein activity, indicating that GusA is indeed a transcription-level reporter system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 19, September 2013, Pages 98-106
Journal: Metabolic Engineering - Volume 19, September 2013, Pages 98-106
نویسندگان
Theresa Siegl, Bogdan Tokovenko, Maksym Myronovskyi, Andriy Luzhetskyy,