کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6550737 | 1421960 | 2018 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantifying chemiluminescence of the forensic luminol test for ovine blood in a dilution and time series
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Quantifying chemiluminescence of the forensic luminol test for ovine blood in a dilution and time series Quantifying chemiluminescence of the forensic luminol test for ovine blood in a dilution and time series](/preview/png/6550737.png)
چکیده انگلیسی
This study investigates the chemiluminescent reaction of whole ovine blood with a luminol solution in a time and dilution series. Replicate samples of both fresh and dried certified pathogen-free ovine blood were prepared and diluted. Seven dilution conditions from neat to 1:1Â 000Â 000 were created for testing. A luminol solution, created using the standard Weber protocol, was applied to all samples in controlled laboratory conditions. A SpectraMax® M3 microplate reader luminometer was used to quantify the chemiluminescence from the reactions as relative luminescence units (RLUs) every four seconds for three minutes. Trends within and amongst the times series, reaction half-lives, and maximum chemiluminescent intensities are discussed. Our research provides a comprehensive dataset derived from instrumental and visual observations on the chemiluminescence resulting from ovine blood's reaction to luminol. This study has implications in forensic bloodstain pattern analysis as it offers a mixed method approach to characterizing the reaction between blood and a commonly used presumptive testing reagent.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International - Volume 290, September 2018, Pages 36-41
Journal: Forensic Science International - Volume 290, September 2018, Pages 36-41
نویسندگان
Sumiko Polacco, Paul Wilson, Mike Illes, Theresa Stotesbury,