کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6551832 | 160416 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Low-template DNA: A single DNA analysis or two replicates?
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
This study investigates the following two questions: (1) Should the DNA analyst concentrate the DNA extract into a single amplification or should he/she split it up to do two replicates? (2) Given the electropherogram obtained from a first analysis, is it worthwhile for the DNA analyst to invest in obtaining a second replicate? A decision-theoretic approach addresses these questions by quantitatively expressing the expected net gain (ENG) of each DNA analysis of interest. The results indicate that two replicates generally have a greater ENG than a single DNA analysis for DNA quantities capable of producing two replicates having an average allelic peak height as low as 43Â rfu. This supports the position that two replicates increase the information content with regard to a single analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International - Volume 264, July 2016, Pages 139-145
Journal: Forensic Science International - Volume 264, July 2016, Pages 139-145
نویسندگان
Simone Gittelson, Carolyn R. Steffen, Michael D. Coble,