کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6579958 | 1422938 | 2018 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Efficient EFGR mediated siRNA delivery to breast cancer cells by Cetuximab functionalized Pluronic® F127/Gelatin
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Efficient EFGR mediated siRNA delivery to breast cancer cells by Cetuximab functionalized Pluronic® F127/Gelatin Efficient EFGR mediated siRNA delivery to breast cancer cells by Cetuximab functionalized Pluronic® F127/Gelatin](/preview/png/6579958.png)
چکیده انگلیسی
New polymeric biomaterials and nanomedicines targeting cancer cells are highly required because of their potential clinical applications. Gene therapy enjoys high popularity as advanced therapy due to its high specificity; however, clinical translation is scarce because the lack of efficient and safe delivery methods. Here we address the design and development of a new nanosized targeting system consisting on Cetuximab-conjugated micelles (PM) of Pluronic® F127 (F127) and Gelatin for efficient delivery of small interfering RNA (siRNA) into epidermal growth factor receptor (EGFR) overexpressing breast cancer cells. Chemical modification by carboxylation of F127 is required prior to conjugation with Cetuximab and PM development. PM presenting appropriate physicochemical features (<40â¯nm) and an efficiently modified surface with Cetuximab were generated. Gelatin garnished the system with the cationic groups (ζâ¯=â¯+30â¯mV) essential for the complexation of siRNA, allowing high entrapment efficiency, and an efficient gene silencing of 70% with low cytotoxicity and proper hemocompatibility. Cetuximab-functionalized PM showed higher rate of cell internalization in EGFR expressing cells than non-functionalized PM, making them a new promising tool for targeted breast cancer treatment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemical Engineering Journal - Volume 340, 15 May 2018, Pages 81-93
Journal: Chemical Engineering Journal - Volume 340, 15 May 2018, Pages 81-93
نویسندگان
Diana Rafael, Francesc MartÃnez, Fernanda Andrade, Joaquin Seras-Franzoso, Natalia Garcia-Aranda, Petra Gener, Joan Sayós, Diego Arango, Ibane Abasolo, Simó Jr.,