کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6582014 456350 2016 37 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Preparation of lipase/Zn3(PO4)2 hybrid nanoflower and its catalytic performance as an immobilized enzyme
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Preparation of lipase/Zn3(PO4)2 hybrid nanoflower and its catalytic performance as an immobilized enzyme
چکیده انگلیسی
A facile and rapid method is reported in this paper to prepare a novel immobilized enzyme named lipase/Zn3(PO4)2 hybrid nanoflower. The growth mechanism of the nanoflower has been studied in detail and can be described as the following four steps: crystallization and coordination, in-situ precipitation, self-assembly, size growth. Addition amount of lipase, reaction temperature and stirring form affect the morphology and lipase content of the hybrid nanoflower. Besides, the catalytic performance of lipase/Zn3(PO4)2 hybrid nanoflower was investigated and the optimal catalytic conditions have been found. The maximum enzyme activity was 855 ± 13 U/g. In comparison with the free lipase, the enzyme activity increment of hybrid nanoflower is 147%. Meanwhile, the unique nanostructure makes lipase/Zn3(PO4)2 hybrid nanoflower an excellent operational stabilizer. The results indicate that the well-designed materials should be useful in industrial enzyme catalysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemical Engineering Journal - Volume 291, 1 May 2016, Pages 287-297
نویسندگان
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