کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6584054 456361 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparison of evaluation methods for Microcystis cell breakage based on dissolved organic carbon release, potassium release and flow cytometry
ترجمه فارسی عنوان
مقایسه روش های ارزیابی شکستگی سلول های میکروکسیستیک بر اساس انتشار کربن آلی حل شده، انتشار پتاسیم و جریان سیاتومتری
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
چکیده انگلیسی
As Microcystis cell breakage may decrease the water quality, the accurate evaluation of cell breakage is of concern in drinking water production. Well-known cell breakage indicators such as dissolved organic carbon (DOC) release and potassium release were investigated in comparison with flow cytometry coupled with a fluorescence probe. DOC release, potassium release and ratios of SYTOX Green positive cells (ruptured cells) were calibrated using known degrees of cell breakage (mixtures of live and heated cells). Good linear relationships were observed between the indicators and the known ratios of ruptured cells, with R2 values larger than 0.925. Flow cytometry coupled with a fluorescence probe had the best overall performance, followed by potassium release and then DOC release. Moreover, the influence of factors such as extracellular organic matter (EOM), cell intake, sample conservation and strong oxidants was also studied. EOM and strong oxidants caused the overestimation and underestimation, respectively, of the cell breakage by DOC release. Potassium release had a more extensive application scope than DOC release and was only influenced by intake by residual live cells. Flow cytometry was generally not affected by EOM, cell intake or strong oxidants except during long-term storage.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemical Engineering Journal - Volume 281, 1 December 2015, Pages 174-182
نویسندگان
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