کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6632439 | 1424950 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An esterase from Penicillium decumbens P6 involved in lignite depolymerization
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کلمات کلیدی
SDSDepolymerizationBHAAHADEAECP/MAS 13C NMR - CP / MAS 13C NMRPenicillium decumbens - decumbens پنی سیلیومEDTA - اتیلن دی آمین تترا استیک اسید ethylene diamine tetraacetic acid - اتیلن دیامین تتراستیک اسیدEsterase - استرسHumic acid - اسید هیومیکSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدSodium dodecyl sulfate polyacrylamide gel electrophoresis - الکتروفورز ژل پلی اتیل آمید سدیم دودسیل سولفاتsodium dodecyl sulfonate - سدیم دودسیل سولفوناتLignite - لیگنیت
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this study, lignite was degraded using a purified esterase from Penicillium decumbens P6 for the first time. The esterase was purified using ammonium sulfate precipitation, anion exchange and gel filtration chromatography. The recovery and purification yield of the enzyme were 15% and 83 folds, respectively. The molecular weight of the purified enzyme was about 45â¯kDa. Both crude and purified esterases were studied for lignite depolymerization. The tendency of depolymerization by crude enzyme was consistent with the enzyme secreted in the medium. Along with the increased purified esterase concentration from 8 to 50â¯mg/ml, A450 value increased from 0.38 to 2.08. The contribution of esterase to the depolymerization was about 40% in the crude supernatant. Compared with aHA (crude lignite humic acid), bHA (esterase degraded lignite humic acid) has a lower percentage of aromatic carbon and ester groups, but a higher percentage of aliphatic carbon. bHA can promote the growth of asparagus lettuce. The results demonstrated that lignite was depolymerized by the purified esterase and evidenced the potential of esterase application in conversion of lignite into compounds with high bioactivities.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fuel - Volume 214, 15 February 2018, Pages 416-422
Journal: Fuel - Volume 214, 15 February 2018, Pages 416-422
نویسندگان
Yi Yang, Jinshui Yang, Baozhen Li, Entao Wang, Hongli Yuan,