کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6662009 1426561 2018 17 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A sensitive biosensor for mercury ions detection based on hairpin hindrance by thymine-Hg(II)-thymine structure
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
A sensitive biosensor for mercury ions detection based on hairpin hindrance by thymine-Hg(II)-thymine structure
چکیده انگلیسی
The exploitation of a dual-signal amplificatory electrochemical DNA biosensor was provided in this article, which can be used to detect Hg(II). One of the signal amplification units is nanocomposite modified electrode and the other is DNA-functionalized Au nanoparticle (DFNP), which include two types of DNA - a hairpin probe DNA with biotin at the 3′ terminal and thiol at the 5′ terminal, and a linear signal DNA with methylene blue (MB)-labeled. Because of the Hg(II) mediated thymine-Hg(II)-thymine (T-Hg(II)-T) structure, when Hg(II) appears, the T-Hg(II)-T that existed between the probe DNA (PD) and helper DNA (HD) can open the hairpin structure of PD and make biotin to be capable of being recognized by avidin, which results in DFNP being brought onto an electrode surface. However, without Hg(II), the distinction between biotin and avidin would not be found. Under optimum conditions, the resultant biosensor brought out a high sensitivity and selectivity for the determination of Hg(II). It has wide linearity from 0.35 pM to 3500 pM (R = 0.9981), and the lower limit of detection is 0.21 pM (S/N = 3). Moreover, the proposed biosensor has been used to its advantage in the test of Hg(II), which was carried out in an environmental water specimen with satisfactory results.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Electroanalytical Chemistry - Volume 814, 1 April 2018, Pages 161-167
نویسندگان
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