The preparation of TiO2/hydroxylapatite (TiO2/HA) composite and sonocatalytic damage to bovine serum albumin (BSA) under ultrasonic irradiation

The TiO2/hydroxylapatite (TiO2/HA) composite with 6.0% HA molar content was prepared by precipitation method and heat-treatment at 500 °C for 40 min. The products were charactered by powder X-ray diffraction (XRD) and scanning electron microscopy (SEM). The sonocatalytic activity of the prepared TiO2/HA composite was studied through the damage of bovine serum albumin (BSA) in aqueous solution under ultrasonic irradiation. The effects of several factors on the sonocatalytic damage of BSA molecules were evaluated by means of UV–vis and fluorescence spectra. It was found that the damage degree was aggravated with the increase of ultrasonic irradiation time, ultrasonic irradiation power, TiO2/HA addition amount, solution acidity and ionic strength. The damage site to BSA molecules was also studied using synchronous fluorescence technology. It was observed that the damage site was mainly focused on the tyrosine (Tyr) residue of BSA molecule. These research results are of great significance for driving sonocatalytic method to treat tumor in clinic application.

TiO2/HA composite was prepared and sonocatalytic activiy was tested through BSA damage. The damage aggravates with increasing irradiation time and the damage site focuses on the tryptophan residue.Figure optionsDownload high-quality image (119 K)Download as PowerPoint slideHighlights
► Coating composite TiO2/hydroxylapatite (TiO2/HA) was prepared by precipitation method.
► TiO2/HA composite is used to study the sonodynamic damage to BSA molecules.
► Binding and damaging sites of TiO2/HA to BSA is studied by synchronous fluorescence technology.
► Some influence factors on the sonodynamic damage to BSA molecules were reviewed.