کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6689881 | 501892 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A thermo- and toxin-tolerant kefir yeast for biorefinery and biofuel production
ترجمه فارسی عنوان
مخمر کربنی مقاوم در برابر حرارت و سموم برای تولید زباله و سوخت زیستی
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی انرژی
مهندسی انرژی و فناوری های برق
چکیده انگلیسی
Although biorefinery has become a common concept to convert biomass into biofuels and value-added chemicals for better cost-performance, good microbial hosts that can be used to implement the concept are still wanting. In this study, a Kluyveromyces marxianus yeast, named KY3, was isolated from a Taiwanese kefir microbial consortium. We showed that KY3 could grow on a broad spectrum of substrates, including hexose and pentose sugars. It is heat and toxin tolerant, can grow under a wide range of pH values (pH 2.5-9), and shows a high ethanol production rate at elevated temperatures. It also can produce value-added aromatic chemicals, such as 2-phenylethylethanol and 2-phenylethyl acetate, during the fermentative process. A genetic transformation was achieved in KY3 to express a rumen fungal β-glucosidase gene, and the transgenic host (KY3-NpaBGS) could efficiently convert cellobiose to ethanol. Furthermore, it was shown that a novel dual-microbe co-culture system of Bacillus subtilis and KY3-NpaBGS can be employed for bioethanol production from cellulosic material. Thus, KY3 has a high potential to be a good host for biorefinery.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Applied Energy - Volume 132, 1 November 2014, Pages 465-474
Journal: Applied Energy - Volume 132, 1 November 2014, Pages 465-474
نویسندگان
Jui-Jen Chang, Cheng-Yu Ho, Chi-Tang Mao, Nathan Barham, Yu-Rong Huang, Feng-Ju Ho, Yueh-Chin Wu, Yu-Han Hou, Ming-Che Shih, Wen-Hsiung Li, Chieh-Chen Huang,