Measurement of solubility of mitomycin C in ethanol–water solutions at different temperatures

Solubility of mitomycin C drug in ethanol–water solutions was measured at different temperatures (0–50 °C) using high performance liquid chromatography. It was observed that the solubility of mitomycin C increases with temperature up to certain concentration of ethanol–water, after that it reduces due to several solute–solvent actions. The process is quite useful for developing nano-crystals of mitomycin C by changing the concentration of co-solvent mixture. From the observations of the present study, it may be suggested that for cardiovascular, ophthamalic and cancer applications of mitomycin C one can use ethanol–water co-solvent mixture for deriving nano-crystals. A modified Apelblat equation was used to correlate the experimental data.

Solubilities of mitomycin C vs. the mole fraction of ethanol (0.0–1.0) on a solute free basis in ethanol–water at different temperatures. (♦) 0 °C, (□) 10 °C, (+) 20 °C, (×) 30 °C, (Δ) 40 °C, (
• ) 50 °C.Figure optionsDownload as PowerPoint slideHighlights
► Solubility of mitomycin C in ethanol–water mixtures (0–50 °C) was measured.
► Modified Apelblat equation was used to correlate the experimental data.
► Solubility increased with temperature upto certain concentration of ethanol–water.
► H2 bonding, polarity, hydrophobic and Van der Waals forces are important.