کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
674611 1459570 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Thermal analysis of cryopreserved Hypericum perforatum L. shoot tips: Cooling regime dependent dehydration and ice growth
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی جریان سیال و فرایندهای انتقال
پیش نمایش صفحه اول مقاله
Thermal analysis of cryopreserved Hypericum perforatum L. shoot tips: Cooling regime dependent dehydration and ice growth
چکیده انگلیسی

It has been well documented that unlike in vitrification during slow cooling at least two major factors, dehydration and ice crystal formation and their growth affect the survival rate of cryopreserved plant material. Presented results with shoot tips of medicinal plant species Hypericum perforatum show negative correlation between cooling rate and recovery after cryopreservation with a maximum of 34.4% at 0.3 °C/min. Dehydration decreases the water content in plant cells and tissues via prolonged exposure time to cryoprotective solutions and/or increased water reduction rate, both at lower cooling rates. Our results indicate no significant influence of cooling-induced dehydration on regeneration capability in samples cooled at different rates. The thermal gradients in the cryo-tube generated during the cooling process represent one of the key parameters of resulting solution‘s thermal behavior. Differential thermal analysis measurements confirm positive correlation between “steepness” of thermal gradients and cooling rates.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Thermochimica Acta - Volume 514, Issues 1–2, 20 February 2011, Pages 22–27
نویسندگان
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