Exploring the inhibitory characteristics of acid hydrolysates upon butanol fermentation: A toxicological assessment

This study aimed to quantitatively evaluate the inhibitor tolerance of butanol-producing bacterium Clostridium acetobutylicum. The inhibitory effect of the inhibitors generated by acid pretreatment of biomass feedstock on butanol fermentation decreased in the order of formic acid > oxalic acid > furfural > 5-HMF > Na2SO4. C. acetobutylicum has a small tolerance range for furfural (1.06-2.6 g/L) and 5-HMF (1.99-2.3 g/L). However, the inhibitory effect of Na2SO4 appears to have a wide range, with a chronic toxicity for C. acetobutylicum. All the results could explain, in quantitative manner, the instability of butanol fermentation with C. acetobutylicum from various acid-pretreated feedstocks caused by the fermentation inhibitors.