کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
679548 1459952 2015 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biomass and lipid production of a local isolate Chlorella sorokiniana under mixotrophic growth conditions
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Biomass and lipid production of a local isolate Chlorella sorokiniana under mixotrophic growth conditions
چکیده انگلیسی


• Our local isolate was identified as Chlorella sorokiniana.
• Our isolate exhibited enhanced growth rate and biomass yield under mixotrophy.
• Nitrogen-starved mixotrophic cells show prominent neutral lipid body accumulation.

A local Chlorella sp. isolate with 97% rbcL sequence identity to Chlorella sorokiniana was evaluated in terms of its biomass and lipid production under mixotrophic growth conditions. Glucose-supplemented cultures exhibited increasing growth rate and biomass yield with increasing glucose concentration. Highest growth rate and biomass yield of 1.602 day−1 and 687.5 mg L−1, respectively, were achieved under 2 g L−1 glucose. Nitrogen starvation up to 75% in the 1.0 g L−1 glucose-supplemented culture was done to induce lipid accumulation and did not significantly affect the growth. Lipid content ranges from 20% to 27% dry weight. Nile Red staining showed more prominent neutral lipid bodies in starved mixotrophic cultures. C. sorokiniana exhibited enhanced biomass production under mixotrophy and more prominent neutral lipid accumulation under nitrogen starvation with no significant decrease in growth; hence, this isolate could be further studied to establish its potential for biodiesel production.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 191, September 2015, Pages 395–398
نویسندگان
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