Immobilized lipase from Schizophyllum commune ISTL04 for the production of fatty acids methyl esters from cyanobacterial oil

• Novel lipase from Schizophyllum commune ISTL04 was immobilized onto Celite.
• Immobilized lipase was studied for transesterification of cyanobacterial oil.
• Crucial reaction conditions were optimized using single factor method.
• Immobilized lipase produced ≈94% FAMEs yield.
• Immobilized lipase produced >90% relative FAMEs yield after 4 repeated cycles.

Novel lipase from model mushroom Schizophyllum commune strain ISTL04 produced by solid state fermentation of Leucaena leucocephala seeds, was immobilized onto Celite for enzymatic FAMEs production from cyanobacterial endolith Leptolyngbya ISTCY101. The isolate showed vigorous growth and produced remarkable lipase activity of 146.5 U g−1 dry solid substrate, without any external lipase inducer. Single-factor experiments were carried out to study the effects of various reaction parameters on the FAMEs yield. The best conditions for enzymatic transesterification as revealed by the results were: 1:3 oil to methanol molar ratio, added at 3 h intervals, 12% water content, 1581.5 U g−1 immobilized lipase, temperature 45 °C, and time 24 h. Under these conditions, the maximum FAMEs yield reached 94%. The immobilized lipase was able to produce >90% of the relative FAMEs yield after four repeated transesterification cycles. This immobilized lipase exhibited potential for application in biodiesel industry.