کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
679831 1459958 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enhanced biomass and oil production from sugarcane bagasse hydrolysate (SBH) by heterotrophic oleaginous microalga Chlorella protothecoides
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Enhanced biomass and oil production from sugarcane bagasse hydrolysate (SBH) by heterotrophic oleaginous microalga Chlorella protothecoides
چکیده انگلیسی


• Sugarcane bagasse hydrolysate (SBH) is a cost-effective and efficient carbon source.
• SBH performed better than glucose for lipid accumulation by C. protothecoides.
• Xylose and arabinose involved in PPP was significant contributor for oil synthesis.
• XYLB, ACAD, and DLD are the metabolic checkpoints in SBH-induced C. protothecoides.

The potential use of sugarcane bagasse hydrolysate (SBH) for microalgal oil in a heterotrophic mode and the oil accumulation mechanisms by SBH-induced Chlorella protothecoides cells were investigated in this study. Results demonstrated that SBH performed better than glucose for cell growth and lipid accumulation under the same reducing sugar concentration. The lipid productivity of 0.69 g/L/d was accomplished at 40 g/L of reducing sugar by batch culture. Under the fed-batch culture condition, the maximum biomass and lipid productivity were 24.01 g/L and 1.19 g/L/d, respectively. Metabolic pathway analysis results indicated that xylose and arabinose involved in pentose phosphate pathway might be predominant over sole glucose involved in glycolysis for lipid accumulation in cells. Three metabolic checkpoints in the proposed metabolic network, including xylulose kinase, acyl-CoA dehydrogenase, and dihydrolipoyl dehydrogenase reveal new possibilities in developing genetic and metabolic engineering microalgae for desirable lipid productivity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 185, June 2015, Pages 99–105
نویسندگان
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