Enhanced production of recombinant aspartase of Aeromonas media NFB-5 in a stirred tank reactor

► Expression conditions for recombinant aspartase from Aeromonas media NFB-5 were optimized. ► Optimization of process parameters for recombinant aspartase production at bioreactor level. ► Permeabilization of recombinant cells to investigate its effect on l-aspartic acid production. ► Permeabilized free recombinant cells and homogenized immobilized cells were compared for l-aspartic acid production.