Optimization of dark fermentative H2 production from microalgal biomass by combined (acid + ultrasonic) pretreatment

In this study, individual pretreatments (acid and ultrasonic) and a combination of these pretreatments were optimized to enhance the efficiency of dark fermentative hydrogen production (DFHP) from microalgal biomass. The experimental results show that the maximum H2 production performance of 42.1 mL H2/g dry cell weight (dcw) was predicted at 0.79% (v/w) HCl and at a specific energy input (SEI) of 49,600 kJ/kg dcw in the combined pretreatment, while it was limited in both individual pretreatments. Repeated batch testing of the predicted optimal conditions revealed that the combined pretreatment conditions for DFHP from microalgal biomass were successfully optimized by increasing the solubilization of the feedstock and by reducing the formation of the toxic 5-hydroxymethylfurfural (HMF).

► H2 production from microalgal biomass by the combined pretreatment.
► Production of 42.1 mL H2/g dry cell weight at 0.79% HCl and at 49,600 kJ/kg dcw.
► Acceleration of H2 yield with less HCl added and a lower specific energy input.
► High solubilization with reduction of 5-hydroxymethylfurfural (HMF) production.